Clinical Chemistry
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Clinical Chemistry 36: 1897-1901, 1990;
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Clinical Chemistry, Vol 36, 1897-1901, Copyright © 1990 by American Association for Clinical Chemistry

Flexibility of noncompetitive avidin-biotin immunoassay: immunoassay of lutropin applied to different signal detection systems

P Vilja
Department of Biomedical Sciences, University of Tampere, Finland.

I adapted the noncompetitive avidin-biotin immunoassay (NABA) of lutropin (LH) for use with radiometric, fluorometric, time-resolved fluorometric, and luminometric detection systems by changing the enzyme substrate or conjugating avidin with different labels. The corresponding methods were used to determine LH in 40 urine samples, and the results were compared with those obtained by an immunoenzymometric assay (IEMA), also based on the NABA principle. Scatter plots of the results showed a linear relation with high correlation coefficients (r = 0.972-0.983), and bias plots showed good agreement between the four comparison methods and the IEMA. The results indicate that the effectiveness of the NABA of LH is independent of the signal detection system. This means good flexibility for the user in choice of instrument and signal detection system.




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Copyright © 1990 by the American Association for Clinical Chemistry.