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Clinical Chemistry, Vol 36, 1928-1933, Copyright © 1990 by American Association for Clinical Chemistry
KS Pettersson and JR Soderholm
Wallac Immunodiagnostic Research Laboratory, Turku, Finland.
A rapid, sensitive two-site immunometric assay for human lutropin (LH), with two monoclonal antibodies directed against the beta subunit, has been developed with time-resolved fluorescence as the detection principle. The cross-reactivities with human thyrotropin and human follitropin are negligible and that with human choriogonadotropin is 1% in the standard two-step protocol. Free beta subunits of LH are also detected. Besides the standard procedure (45 + 15 min), more rapid alternative protocols (15 + 15 min or 30 min in one step) have also been evaluated. The minimum detectable dose per well is approximately 1 amol (10(-18) mol), corresponding to less than 0.01 int. unit/L, for a sample volume of 25 microL and less than 0.001 int. unit/L for 200- microL samples. The intra- and interassay CV is less than 6% for LH concentrations from 0.2 to 250 int. units/L. With 200-microL sample volumes, intra-assay variation is less than 6% at 0.04 int. unit/L and interassay variation is 7% at 0.05 int. unit/L. The very high sensitivity and reproducibility of the presented method enables assay of low prepubertal and of suppressed LH concentrations, and represents a clear improvement over currently available immunoassays.
The following articles in journals at HighWire Press have cited this article:
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Q.-P. Qin, M. Christiansen, and K. Pettersson Point-of-Care Time-resolved Immunofluorometric Assay for Human Pregnancy-associated Plasma Protein A: Use in First-Trimester Screening for Down Syndrome Clin. Chem., March 1, 2002; 48(3): 473 - 483. [Abstract] [Full Text] [PDF]
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V. N. Brito, M. C. Batista, M. F. Borges, A. C. Latronico, M. B. F. Kohek, A. C. P. Thirone, B. H. Jorge, I. J. P. Arnhold, and B. B. Mendonca Diagnostic Value of Fluorometric Assays in the Evaluation of Precocious Puberty J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3539 - 3544. [Abstract] [Full Text] [PDF]
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A. W. van den Beld, I. T. Huhtaniemi, K. S. L. Pettersson, H. A. P. Pols, D. E. Grobbee, F. H. de Jong, and S. W. J. Lamberts Luteinizing Hormone and Different Genetic Variants, as Indicators of Frailty in Healthy Elderly Men J. Clin. Endocrinol. Metab., April 1, 1999; 84(4): 1334 - 1339. [Abstract] [Full Text]
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A. Eskola-Williams and G. J. Fagan Misleading Results with Opus® Magnum hLH Assay • A spokesman from Behring replies: Clin. Chem., October 1, 1997; 43(10): 2008 - 2010. [Full Text]
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D. M. Robertson, N. Cahir, J. K. Findlay, H. G. Burger, and N. Groome The Biological and Immunological Characterization of Inhibin A and B Forms in Human Follicular Fluid and Plasma J. Clin. Endocrinol. Metab., March 1, 1997; 82(3): 889 - 896. [Abstract] [Full Text] [PDF]
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