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Clinical Chemistry 36: 1934-1936, 1990;
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Clinical Chemistry, Vol 36, 1934-1936, Copyright © 1990 by American Association for Clinical Chemistry

Particulate (high-molecular-mass) and soluble alkaline phosphatase in urine determined by high-performance liquid chromatography

E Schoenau, KH Herzog and D Michalk
Klinik fur Kinder und Jugendliche der Friedrich-Alexander Universitat, Erlangen-Nurnberg, F.R.G.

We detected in urine by HPLC two enzyme fractions of alkaline phosphatase (AP, EC 3.1.3.1), soluble and particulate, analogous to those in serum. The second fraction was eluted with high-salt-content eluent at the same elution time as high-molecular-mass, or particulate, AP in serum. AP characterization in urine from a patient with acute rejection crisis showed a greater sensitivity of the particulate form to treatment with L-phenylalanine, which suggests a higher content of intestinal-type AP in the particulate form. The soluble fraction showed a more liver-type AP behavior. Changes in the chromatograms after the sample was treated with 1-butanol and Triton X-100 support a membrane origin of the particulate AP. Urinalyses from patients with acute renal disease showed increased activity of soluble and particulate AP, with a relatively greater increase of particulate AP.




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Copyright © 1990 by the American Association for Clinical Chemistry.