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Clinical Chemistry 36: 1937-1940, 1990;
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Clinical Chemistry, Vol 36, 1937-1940, Copyright © 1990 by American Association for Clinical Chemistry

Enzymatic method for selective determination of 4-aminobenzoic acid in urine

N Bando, T Ogawa, H Tsuji and K Sasaoka
Department of Nutrition, School of Medicine, University of Tokushima, Japan.

We developed a method for the selective determination of 4-aminobenzoic acid by use of 4-aminobenzoate hydroxylase (EC 1.14.13.27) to convert 4- aminobenzoic acid to 4-hydroxyaniline. The subsequent conversion of 4- hydroxyaniline to indophenol dye was quantified colorimetrically. The method is reproducible, and the assay response is linear up to 40 mumol/L. Selectivity exceeding that of the current colorimetric assays was demonstrated for these enzymatic determinations of 4-aminobenzoic acid concentrations in urine samples from patients undergoing tests of pancreatic function with bentiromide. This method effectively minimizes interferences from drugs and diet, a problem in current colorimetric methods.




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Copyright © 1990 by the American Association for Clinical Chemistry.