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Clinical Chemistry 36: 1941-1944, 1990;
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Clinical Chemistry, Vol 36, 1941-1944, Copyright © 1990 by American Association for Clinical Chemistry

Heterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol

Y Xu, B Halsall and WR Heineman
Department of Chemistry, University of Cincinnati, OH 45221-0172.

A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.


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S.-F. Chen, Y. Xu, and M. P.-C. Ip
Electrochemical Enzyme Immunoassay for Serum Prostate-Specific Antigen at Low Concentrations
Clin. Chem., August 1, 1997; 43(8): 1459 - 1461.
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