Clinical Chemistry
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Clinical Chemistry 36: 1956-1957, 1990;
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Clinical Chemistry, Vol 36, 1956-1957, Copyright © 1990 by American Association for Clinical Chemistry

Analysis of 8-methoxypsoralen by high-performance liquid chromatography

CH Ketchum, CA Robinson Jr and ST Huang
Department of Pathology, University of Alabama, Birmingham 35294.

We report a simple and rapid procedure for assaying 8-methoxypsoralen (8-MOP) in plasma by high-performance liquid chromatography (HPLC). The standard curve for the assay is linear for 8-MOP from 15 to 500 micrograms/L (y = 0.002x-0.01, r = 0.99) with a lower limit of detection of 1.5 micrograms/L. Intra-assay precision (CV) was 6.0% at the 100 micrograms/L concentration and 10.0% at 50 micrograms/L (n = 30 each). Interassay precision was 6.4% at 100 micrograms/L and 7.0% at 50 micrograms/L (n = 50 each). Extraction recovery of 8-MOP was 98%. Common antiarrhythmics, sedatives, and hypnotics were found not to interfere.


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Copyright © 1990 by the American Association for Clinical Chemistry.