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Clinical Chemistry 36: 1964-1966, 1990;
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Clinical Chemistry, Vol 36, 1964-1966, Copyright © 1990 by American Association for Clinical Chemistry

Inhibition of lactate dehydrogenase isoenzymes by sodium perchlorate evaluated

GT Sanders, E van der Neut and JP van Straalen
Department of Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands.

We evaluated a method of measuring lactate dehydrogenase isoenzyme 1 (LD-1) selectively (Clin Chem 1987;33:991-2), in which all other LD isoenzymes were inhibited by adding sodium perchlorate to the reaction medium to a final concentration of 0.825 mol/L. In this study we used the different isoenzymes purified from human autopsy tissue and found that the originally published amount of inhibitor (a) increased the original LD-1 activity and (b) did not eliminate all enzyme activity of LD-2 and LD-3. Interference by LD-2 was further demonstrated. Thus we conclude that this method cannot be used for the selective determination of LD-1 because its results do not accurately reflect the original LD-1 activity.




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Copyright © 1990 by the American Association for Clinical Chemistry.