Clinical Chemistry
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Clinical Chemistry 36: 2036-2041, 1990;
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Clinical Chemistry, Vol 36, 2036-2041, Copyright © 1990 by American Association for Clinical Chemistry

Direct chemiluminescence immunoassay of estradiol in saliva

J De Boever, F Kohen, J Bouve, D Leyseele and D VandeKerckhove
Department of Obstetrics and Gynecology, University Hospital, Gent, Belgium.

A sensitive and simple direct solid-phase chemiluminescence immunoassay is described for estradiol in saliva. In this assay, a second antibody is bound to the wells of microtiter plates. Either buffer with standards or saliva (100 microL) is incubated in these wells with monoclonal anti-estradiol antibody and with estradiol-isoluminol conjugate. Incubation time is 2 h. Chemiluminescence of the bound fraction is measured in a manually operated luminometer (Biocounter). The assay has a detection limit of 3.8 pmol/L; analytical recovery of added estradiol is 96.8% (SD 7.0%); within- and between-assay CVs range between 2.5% and 12.7%. Forty unknown saliva samples can be assayed and results calculated within 4.5 h. Results of a slightly modified procedure-with black microtiter plates and a prototype of an automated plate reader (Luminoskan)--compare well with those of the described method (r = 0.97). Because steroid-binding globulins have been found in saliva, the effect of displacing agents on the results of the direct chemiluminescence assay is described.


The following articles in journals at HighWire Press have cited this article:


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K. Tamate, M. Charleton, J. P. Gosling, D. Egan, M. Ishikawa, P. F. Fottrell, and M. M. Kane
Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17{beta} in saliva
Clin. Chem., July 1, 1997; 43(7): 1159 - 1164.
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