Clinical Chemistry
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Clinical Chemistry 36: 2063-2071, 1990;
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Clinical Chemistry, Vol 36, 2063-2071, Copyright © 1990 by American Association for Clinical Chemistry

Automation of specific human gene detection

PE Mayrand, LB Hoff, LJ McBride, JA Bridgham, R Cathcart, KP Corcoran, GS Golda, DH Keith, EW Lachenmeier and DE Madden
Applied Biosystems, Inc., Foster City, CA 94404.

An instrument/chemistry system is described that automates a new chemical procedure functionally equivalent to Southern blotting. A fluorescence gel scanner that detects migrating DNA fragments in real- time analyzes the samples produced by a prototype liquid-handling instrument that automates a solution-phase hybridization/solid-phase capture chemistry for DNA analysis. The combination of this chemistry, the gel scanner, and robotic automation eliminates the tedium encountered in traditional manual methods for specific gene detection and reduces analysis time from days to hours. Restriction fragment lengths are measured with high precision by comparison with in-lane standards to minimize effects attributable to migration anomalies. The utility of this automated system is demonstrated by executing a clinical research application involving hybridization to a multi-copy repeat sequence on the Y chromosome and its detection.




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Copyright © 1990 by the American Association for Clinical Chemistry.