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Clinical Chemistry, Vol 36, 2124-2126, Copyright © 1990 by American Association for Clinical Chemistry
TM Sheehan and M Gao
West Midlands Regional Laboratory for Toxicology, Dudley Road Hospital, Birmingham, U.K.
We describe here a single-tube assay that may be applied to the whole range of selenium status in adult and pediatric patients, including depletion during parenteral or other nutrition. A specimen or aqueous standard, 100 microL, is digested with 0.5 mL of HNO3/HCIO4 (4/1 by vol, at 190 degrees C for 90 min), reduced with 0.5 mL of concentrated HCI (150 degrees C, 30 min), and complexed with 0.5 mL of 6.3 mmol/L 2,3-diaminonaphthalene (DAN) reagent in the presence of EDTA (60 degrees C, 30 min). The resulting fluorophore is extracted into cyclohexane and its fluorescence measured (excitation at 366 nm; emission at 544 nm). It is not necessary to control pH during the complexing step or to protect the DAN from light. The limit of detection of selenium is 10 micrograms/L (0.126 mumol/L); linearity of results extends to 2000 micrograms/L (25.3 mumol/L). Between-batch precision is 5%, analytical recovery 90%-96%. Performance is good as tested against Reference Materials and by participation in a National Quality Assurance Scheme.
The following articles in journals at HighWire Press have cited this article:
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  R. F. Burk, K. E. Hill, G. E. Olson, E. J. Weeber, A. K. Motley, V. P. Winfrey, and L. M. Austin Deletion of Apolipoprotein E Receptor-2 in Mice Lowers Brain Selenium and Causes Severe Neurological Dysfunction and Death When a Low-Selenium Diet Is Fed J. Neurosci., June 6, 2007; 27(23): 6207 - 6211. [Abstract] [Full Text] [PDF]
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 G. E. Olson, V. P. Winfrey, S. K. NagDas, K. E. Hill, and R. F. Burk Apolipoprotein E Receptor-2 (ApoER2) Mediates Selenium Uptake from Selenoprotein P by the Mouse Testis J. Biol. Chem., April 20, 2007; 282(16): 12290 - 12297. [Abstract] [Full Text] [PDF]
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K. E. Hill, J. Zhou, L. M. Austin, A. K. Motley, A.-J. L. Ham, G. E. Olson, J. F. Atkins, R. F. Gesteland, and R. F. Burk The Selenium-rich C-terminal Domain of Mouse Selenoprotein P Is Necessary for the Supply of Selenium to Brain and Testis but Not for the Maintenance of Whole Body Selenium J. Biol. Chem., April 13, 2007; 282(15): 10972 - 10980. [Abstract] [Full Text] [PDF]
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 A. Nakayama, K. E. Hill, L. M. Austin, A. K. Motley, and R. F. Burk All Regions of Mouse Brain Are Dependent on Selenoprotein P for Maintenance of Selenium J. Nutr., March 1, 2007; 137(3): 690 - 693. [Abstract] [Full Text] [PDF]
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 R. F. Burk, B. K. Norsworthy, K. E. Hill, A. K. Motley, and D. W. Byrne Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial. Cancer Epidemiol. Biomarkers Prev., April 1, 2006; 15(4): 804 - 810. [Abstract] [Full Text] [PDF]
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 Y. Xia, K. E Hill, D. W Byrne, J. Xu, and R. F Burk Effectiveness of selenium supplements in a low-selenium area of China Am. J. Clinical Nutrition, April 1, 2005; 81(4): 829 - 834. [Abstract] [Full Text] [PDF]
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K. E. Hill, J. Zhou, W. J. McMahan, A. K. Motley, J. F. Atkins, R. F. Gesteland, and R. F. Burk Deletion of Selenoprotein P Alters Distribution of Selenium in the Mouse J. Biol. Chem., April 11, 2003; 278(16): 13640 - 13646. [Abstract] [Full Text] [PDF]
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