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Clinical Chemistry 36: 2131-2133, 1990;
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Clinical Chemistry, Vol 36, 2131-2133, Copyright © 1990 by American Association for Clinical Chemistry

Semi-automated colorimetric method for measuring glycohemoglobin, with reduction of nitroblue tetrazolium, evaluated

RP Hill
Department of Biochemistry, King's Mill Hospital, Sutton-in-Ashfield, Nottinghamshire, U.K.

I evaluated a semi-automated method for measuring glycohemoglobin via reduction of nitroblue tetrazolium by acetone-extracted globin. The simultaneous measurement of glycated protein and total protein with the Roche Mira S analyzer showed good analytical precision (between-batch CV 3.5%-8.5%). Correlation of results (y) with those by a boronate affinity chromatography method (x) gave a regression line of y = 2.0x + 5.9 for samples from patients attending a diabetic outpatient clinic. I also studied reaction kinetics; chloromercuribenzoate concentration, sample volume, and detergent concentration affect the reaction, probably by modulating the reduction of dye by globin-thiol groups.




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Copyright © 1990 by the American Association for Clinical Chemistry.