Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 36: 271-276, 1990;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bouillon, R.
Right arrow Articles by Eliard, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bouillon, R.
Right arrow Articles by Eliard, P. H.

Clinical Chemistry, Vol 36, 271-276, Copyright © 1990 by American Association for Clinical Chemistry

Immunoradiometric assay of parathyrin with polyclonal and monoclonal region-specific antibodies

R Bouillon, W Coopmans, DE Degroote, D Radoux and PH Eliard
Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium.

In this immunoradiometric assay (IRMA) of parathyrin (PTH) a polyclonal anti-amino-PTH(1-34) is the capture antibody and a radiolabeled monoclonal anti-hPTH(44-68) is the second antibody. Gel filtration of serum from a hyperparathyroid patient yielded only a single peak of PTH, corresponding to the elution position of synthetic PTH(1-84). Healthy elderly individuals (ages 78 +/- 5 y, mean +/- SD, n = 45) had PTH concentrations (21 +/- 13 ng/L) not significantly higher than those from healthy younger (38 +/- 11 y) adults (20 +/- 8 ng/L, n = 94). Assay results agreed well with those obtained with a carboxyl-terminal PTH assay both in normal subjects (r = 0.63, P less than 0.001) and in patients with primary hyperparathyroidism (r = 0.59, P less than 0.001). Both assays equally discriminated patients with surgically confirmed primary hyperparathyroidism from normal individuals, but the PTH(1-84) IRMA also allowed a nearly absolute discrimination between normal subjects and patients with primary hypoparathyroidism (undetectable serum PTH in 18 of 21 cases) and secondary hypoparathyroidism (caused by hypercalcemia that was caused by a malignant tumor, PTH 1.3 +/- 1.3 ng/L, n = 32). Moreover, the PTH(1-84) IRMA is more sensitive (detection limit in serum, 0.8 ng/L) and easier and quicker to perform than the carboxyl-terminal assay.


The following articles in journals at HighWire Press have cited this article:


Home page
 CJASNHome page
P. Evenepoel, B. Van Den Bergh, M. Naesens, H. De Jonge, B. Bammens, K. Claes, D. Kuypers, and Y. Vanrenterghem
Calcium Metabolism in the Early Posttransplantation Period
Clin. J. Am. Soc. Nephrol., March 1, 2009; 4(3): 665 - 672.
[Abstract] [Full Text] [PDF]

Home page
 CJASNHome page
P. Evenepoel, B. K.I. Meijers, H. de Jonge, M. Naesens, B. Bammens, K. Claes, D. Kuypers, and Y. Vanrenterghem
Recovery of Hyperphosphatoninism and Renal Phosphorus Wasting One Year after Successful Renal Transplantation
Clin. J. Am. Soc. Nephrol., November 1, 2008; 3(6): 1829 - 1836.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
P. Evenepoel, K. Claes, D. R. Kuypers, F. Debruyne, and Y. Vanrenterghem
Parathyroidectomy after successful kidney transplantation: a single centre study
Nephrol. Dial. Transplant., June 1, 2007; 22(6): 1730 - 1737.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
P. Evenepoel, K. Claes, D. Kuypers, B. Maes, and Y. Vanrenterghem
Impact of parathyroidectomy on renal graft function, blood pressure and serum lipids in kidney transplant recipients: a single centre study
Nephrol. Dial. Transplant., August 1, 2005; 20(8): 1714 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
P. Evenepoel, K. Claes, D. Kuypers, B. Maes, B. Bammens, and Y. Vanrenterghem
Natural history of parathyroid function and calcium metabolism after kidney transplantation: a single-centre study
Nephrol. Dial. Transplant., May 1, 2004; 19(5): 1281 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. Amin, A. Fawzy, M. A. Hamid, and A. Elhendy
Pulmonary Hypertension in Patients With Chronic Renal Failure: Role of Parathyroid Hormone and Pulmonary Artery Calcifications
Chest, December 1, 2003; 124(6): 2093 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Van den Berghe, D. Van Roosbroeck, P. Vanhove, P. J. Wouters, L. De Pourcq, and R. Bouillon
Bone Turnover in Prolonged Critical Illness: Effect of Vitamin D
J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4623 - 4632.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Van den Berghe, F. Weekers, R. C. Baxter, P. Wouters, A. Iranmanesh, R. Bouillon, and J. D. Veldhuis
Five-Day Pulsatile Gonadotropin-Releasing Hormone Administration Unveils Combined Hypothalamic-Pituitary-Gonadal Defects Underlying Profound Hypoandrogenism in Men with Prolonged Critical Illness
J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3217 - 3226.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Fournier, M. E. C. Solal, R. Oprisiu, H. Mazouz, P. Morinire, G. Choukroun, and R. Bouillon
Optimal Range of Plasma Concentration of True 1-84 Parathyroid Hormone in Patients on Maintenance Dialysis
J. Clin. Endocrinol. Metab., April 1, 2001; 86(4): 1840 - 1841.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Association for Clinical Chemistry.