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Clinical Chemistry, Vol 36, 271-276, Copyright © 1990 by American Association for Clinical Chemistry
R Bouillon, W Coopmans, DE Degroote, D Radoux and PH Eliard
Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium.
In this immunoradiometric assay (IRMA) of parathyrin (PTH) a polyclonal anti-amino-PTH(1-34) is the capture antibody and a radiolabeled monoclonal anti-hPTH(44-68) is the second antibody. Gel filtration of serum from a hyperparathyroid patient yielded only a single peak of PTH, corresponding to the elution position of synthetic PTH(1-84). Healthy elderly individuals (ages 78 +/- 5 y, mean +/- SD, n = 45) had PTH concentrations (21 +/- 13 ng/L) not significantly higher than those from healthy younger (38 +/- 11 y) adults (20 +/- 8 ng/L, n = 94). Assay results agreed well with those obtained with a carboxyl-terminal PTH assay both in normal subjects (r = 0.63, P less than 0.001) and in patients with primary hyperparathyroidism (r = 0.59, P less than 0.001). Both assays equally discriminated patients with surgically confirmed primary hyperparathyroidism from normal individuals, but the PTH(1-84) IRMA also allowed a nearly absolute discrimination between normal subjects and patients with primary hypoparathyroidism (undetectable serum PTH in 18 of 21 cases) and secondary hypoparathyroidism (caused by hypercalcemia that was caused by a malignant tumor, PTH 1.3 +/- 1.3 ng/L, n = 32). Moreover, the PTH(1-84) IRMA is more sensitive (detection limit in serum, 0.8 ng/L) and easier and quicker to perform than the carboxyl-terminal assay.
The following articles in journals at HighWire Press have cited this article:
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  P. Evenepoel, K. Claes, D. R. Kuypers, F. Debruyne, and Y. Vanrenterghem Parathyroidectomy after successful kidney transplantation: a single centre study Nephrol. Dial. Transplant., June 1, 2007; 22(6): 1730 - 1737. [Abstract] [Full Text] [PDF]
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P. Evenepoel, K. Claes, D. Kuypers, B. Maes, and Y. Vanrenterghem Impact of parathyroidectomy on renal graft function, blood pressure and serum lipids in kidney transplant recipients: a single centre study Nephrol. Dial. Transplant., August 1, 2005; 20(8): 1714 - 1720. [Abstract] [Full Text] [PDF]
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P. Evenepoel, K. Claes, D. Kuypers, B. Maes, B. Bammens, and Y. Vanrenterghem Natural history of parathyroid function and calcium metabolism after kidney transplantation: a single-centre study Nephrol. Dial. Transplant., May 1, 2004; 19(5): 1281 - 1287. [Abstract] [Full Text] [PDF]
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 M. Amin, A. Fawzy, M. A. Hamid, and A. Elhendy Pulmonary Hypertension in Patients With Chronic Renal Failure: Role of Parathyroid Hormone and Pulmonary Artery Calcifications Chest, December 1, 2003; 124(6): 2093 - 2097. [Abstract] [Full Text] [PDF]
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 G. Van den Berghe, D. Van Roosbroeck, P. Vanhove, P. J. Wouters, L. De Pourcq, and R. Bouillon Bone Turnover in Prolonged Critical Illness: Effect of Vitamin D J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4623 - 4632. [Abstract] [Full Text] [PDF]
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G. Van den Berghe, F. Weekers, R. C. Baxter, P. Wouters, A. Iranmanesh, R. Bouillon, and J. D. Veldhuis Five-Day Pulsatile Gonadotropin-Releasing Hormone Administration Unveils Combined Hypothalamic-Pituitary-Gonadal Defects Underlying Profound Hypoandrogenism in Men with Prolonged Critical Illness J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3217 - 3226. [Abstract] [Full Text] [PDF]
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A. Fournier, M. E. C. Solal, R. Oprisiu, H. Mazouz, P. Morinire, G. Choukroun, and R. Bouillon Optimal Range of Plasma Concentration of True 1-84 Parathyroid Hormone in Patients on Maintenance Dialysis J. Clin. Endocrinol. Metab., April 1, 2001; 86(4): 1840 - 1841. [Full Text]
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