Clinical Chemistry
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Clinical Chemistry 36: 277-282, 1990;
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Clinical Chemistry, Vol 36, 277-282, Copyright © 1990 by American Association for Clinical Chemistry

Development of an enzyme-linked immunosorbent assay with a monoclonal antibody prepared against alpha 1-antitrypsin for diagnostic screening of inflammatory disorders

B Silvestrini, A Guglielmotti, L Saso, C Milanese, E Melanitou, J Grima and CY Cheng
Institute of Pharmacology and Pharmacognosy, University of Rome, Italy.

A monoclonal antibody, designated A2a18b8, of IgG1 class prepared against human alpha 1-antitrypsin, cross-reacts with alpha 1- antitrypsin in the serum of rat and baboon, but not with alpha 1- antitrypsin in serum of rabbit, pig, hamster, guinea pig, dog, or turtle. We used A2a18b8 in an enzyme-linked immunosorbent assay (ELISA) developed for human alpha 1-antitrypsin. Preliminary ELISA screening of 247 serum samples from patients with various inflammatory disorders indicated that the concentration of a specific epitope(s) on alpha 1- antitrypsin recognized by this monoclonal antibody was increased significantly in patients with active systemic lupus erythematosus, mixed connective tissue disease, and rheumatoid arthritis, but not in patients with sclerodermic disorders or Sjogren's syndrome. Evidently, A2a18b8 has diagnostic value in that it selectively recognizes a specific epitope(s) on alpha 1-antitrypsin that is (are) apparently exposed during selective inflammatory disorders.


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