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Clinical Chemistry 36: 290-296, 1990;
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Clinical Chemistry, Vol 36, 290-296, Copyright © 1990 by American Association for Clinical Chemistry

Effects of analytical method and lyophilized sera on measurements of apolipoproteins A-I and B: an international survey

SJ Smith, LO Henderson, WH Hannon and GR Cooper
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.

In 1987 a collaborative study was initiated with 140 laboratories worldwide to evaluate the effects of analytical method and lyophilization on the measurement of different concentrations of apolipoproteins (apo) A-I and B in four lyophilized serum pool samples. This survey confirmed that the lyophilized apo Reference Material of the International Union of Immunological Societies (IUIS) is useful for apo A-I assays as an international serum-based reference material, because among-method variation is negligible. The apo A-I concentration value of 1.24 g/L is now assigned to the IUIS Reference Material (CDC 1883) by a Centers for Disease Control RIA in-house reference method. Use of lyophilized serum preparation as a reference material for some modes of apo B measurement is questionable because of lyophilization and matrix effects. Both radial immunodiffusion and liquid immunoprecipitin methods demonstrated bias in measured apo B concentrations, compared with overall method-weighted means values on the IUIS Reference Material. Because of the uncertainty associated with LDL primary standard, protein analysis, and concentration differences among analytical methods, assigning a single apo B concentration value to the IUIS Reference Material appears inadvisable at present.




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Copyright © 1990 by the American Association for Clinical Chemistry.