Clinical Chemistry
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Clinical Chemistry 36: 331-333, 1990;
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Clinical Chemistry, Vol 36, 331-333, Copyright © 1990 by American Association for Clinical Chemistry

Simple enzymatic assay for determining cholesterol concentrations in bile

CM Luhman, ST Galloway and DC Beitz
Department of Animal Science, Iowa State University, Ames 50011.

We use bilirubin oxidase (EC 1.3.3.5) to remove interference by bilirubin in the assay of cholesterol concentration in bile by standard enzymatic methods. Samples are treated for 10 min with nonlimiting amounts of bilirubin oxidase to form biliverdin from bilirubin before the reagent for cholesterol is added. The relatively small interference by biliverdin is easily eliminated by use of sample blanks. The method is simple, convenient, and not hampered by the "chromogen oxidase" activity (the inherent ability of bilirubin oxidase to oxidize some chromogens) that plagues other assays of this type. Using this assay, we have accurately and precisely determined the concentration of cholesterol in bile. Such elimination of bilirubin will also be useful in assays of other biliary constituents or constituents of urine or icteric plasma.


The following articles in journals at HighWire Press have cited this article:


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D. Masson, B. Staels, T. Gautier, C. Desrumaux, A. Athias, N. Le Guern, M. Schneider, Z. Zak, L. Dumont, V. Deckert, et al.
Cholesteryl ester transfer protein modulates the effect of liver X receptor agonists on cholesterol transport and excretion in the mouse
J. Lipid Res., March 1, 2004; 45(3): 543 - 550.
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