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Clinical Chemistry, Vol 36, 340-344, Copyright © 1990 by American Association for Clinical Chemistry
H Landy, AL Schneyer, RW Whitcomb and WF Crowley Jr
Department of Medicine, Children's Hospital Medical Center, Boston, MA 02115.
Measurement of the urinary excretion of lutropin (LH) and follitropin (FSH) and their common free alpha subunit (FAS) assists in monitoring the maturation of the hypothalamic-pituitary-gonadal axis and in understanding the physiology of the pituitary glycoprotein hormones. Here we describe sensitive, specific polyclonal radioimmunoassays for LH and FSH and a monoclonal radioimmunoassay for FAS for use with urine- -assays unperturbed by alterations in urinary pH or osmolarity within the broad physiological range encountered in urine. Concordance between LH, FSH, and FAS concentrations in extracted and unextracted urine samples was high. Linearity and parallelism with the standard curves was observed with addition of 25 to 200 microL of unextracted urine. No effect on glycoprotein concentration was seen after as many as 10 freeze-thaw cycles. The need for extraction was further obviated by the high sensitivity of each assay, reflected by minimum detectable doses well below the concentrations encountered in patients' samples. Thus we have measured gonadotropins in unextracted urine as precisely as in extracted urine. We also have demonstrated an equally versatile assay for urinary alpha subunit, using a monoclonal antibody of high specificity for this monomer in its free, uncombined form. These radioimmunoassays complement assays of gonadotropins and free alpha subunit in serum and will allow longitudinal investigations otherwise limited by the constraints of the patient's blood volume.
The following articles in journals at HighWire Press have cited this article:
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  C. K. Welt, Y. L. Pagan, P. C. Smith, K. B. Rado, and J. E. Hall Control of Follicle-Stimulating Hormone by Estradiol and the Inhibins: Critical Role of Estradiol at the Hypothalamus during the Luteal-Follicular Transition J. Clin. Endocrinol. Metab., April 1, 2003; 88(4): 1766 - 1771. [Abstract] [Full Text] [PDF]
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F. J. Hayes, N. Pitteloud, S. DeCruz, W. F. Crowley Jr., and P. A. Boepple Importance of Inhibin B in the Regulation of FSH Secretion in the Human Male J. Clin. Endocrinol. Metab., November 1, 2001; 86(11): 5541 - 5546. [Abstract] [Full Text] [PDF]
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F. J. Hayes, D. J. McNicholl, D. Schoenfeld, E. E. Marsh, and J. E. Hall Free {alpha}-Subunit Is Superior to Luteinizing Hormone as a Marker of Gonadotropin-Releasing Hormone Despite Desensitization at Fast Pulse Frequencies J. Clin. Endocrinol. Metab., March 1, 1999; 84(3): 1028 - 1036. [Abstract] [Full Text]
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H. B. Lavoie, K. A. Martin, A. E. Taylor, W. F. Crowley, and J. E. Hall Exaggerated Free {alpha}-Subunit Levels during Pulsatile Gonadotropin-Releasing Hormone Replacement in Women with Idiopathic Hypogonadotropic Hypogonadism J. Clin. Endocrinol. Metab., January 1, 1998; 83(1): 241 - 247. [Abstract] [Full Text]
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 U.-H. Stenman, L. Unkila-Kallio, J. Korhonen, and H. Alfthan Immunoprocedures for detecting human chorionic gonadotropin: clinical aspects and doping control Clin. Chem., July 1, 1997; 43(7): 1293 - 1298. [Abstract] [Full Text] [PDF]
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