Clinical Chemistry
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Clinical Chemistry 36: 348-350, 1990;
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Clinical Chemistry, Vol 36, 348-350, Copyright © 1990 by American Association for Clinical Chemistry

Mitochondrial aspartate aminotransferase determined by "Fast Protein Liquid Chromatography"

M Grostad, R Rej and NE Huseby
Institute of Medical Biology, University of Tromso, Norway.

We describe an improved separation of the isoenzymes of aspartate aminotransferase (EC 2.6.1.1), based on ion-exchange chromatography. Involving the "Fast Protein Liquid Chromatography" system (Pharmacia) with a MonoQ column, this rapid, reproducible method for quantifying the mitochondrial enzyme shows good resolution and sensitivity, and results correlate well with those by an established immunochemical method.




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