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Clinical Chemistry 36: 370-375, 1990;
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Clinical Chemistry, Vol 36, 370-375, Copyright © 1990 by American Association for Clinical Chemistry

A comparison of results for cholesterol in human serum obtained by the Reference Method and by the Definitive Method of the National Reference System for cholesterol

P Ellerbe, GL Myers, GR Cooper, HS Hertz, LT Sniegoski, MJ Welch and E White 5th
Organic Analytical Research Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.

Here we compare the Reference Method (I) and the Definitive Method (II) for measurement of cholesterol in serum. For pure cholesterol solutions, values by I agree with values by II, but for fresh, frozen, or lyophilized sera, values by I average 1.6% higher than values by II. We conclude that an undetected interference is associated with I. However, the observed difference does not diminish the usefulness of I as an accuracy base for cholesterol measurements, because it is clinically insignificant at present.


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