Clinical Chemistry
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Clinical Chemistry 36: 446-449, 1990;
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Clinical Chemistry, Vol 36, 446-449, Copyright © 1990 by American Association for Clinical Chemistry

Rapid, robust method for measuring low concentrations of albumin in urine

EA Medcalf, DJ Newman, EG Gorman and CP Price
Department of Clinical Biochemistry, London Hospital Medical College, U.K.

We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.


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