Clinical Chemistry
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Clinical Chemistry 36: 466-469, 1990;
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Clinical Chemistry, Vol 36, 466-469, Copyright © 1990 by American Association for Clinical Chemistry

Neutral ionophore-based selective electrode for assaying the activity of magnesium in undiluted blood serum

M Rouilly, B Rusterholz, UE Spichiger and W Simon
Department of Organic Chemistry, Swiss Federal Institute of Technology, Zurich, Switzerland.

The concentration of free, ionized magnesium in undiluted blood serum can be determined potentiometrically with a new magnesium-selective carrier in polymeric membrane electrodes (by weight 66% plasticizer, approximately 33% polyvinyl chloride and approximately 1% ionophore). However, discrimination of calcium by the new ionophore-added membrane is not sufficient to keep calcium interference to less than 1%. To correct for the interference by calcium, we determine the calcium activity of the serum sample with a calcium-selective electrode and calibrate the magnesium-selective electrode with standard solutions containing calcium in the concentrations found. In this way we determined the free ionized magnesium in five plasma samples from five different persons and found it to be within the range expected for magnesium in undiluted serum.


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L. M. Thienpont, K. Dewitte, and D. Stockl
Serum complexed magnesium--a cautionary note on its estimation and its relevance for standardizing serum ionized magnesium.
Clin. Chem., January 1, 1999; 45(1): 154 - 155.
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H. J. Huijgen, R. Sanders, R. W. van Olden, M. G. Klous, F. R. Gaffar, and G. T. B. Sanders
Intracellular and extracellular blood magnesium fractions in hemodialysis patients; is the ionized fraction a measure of magnesium excess?
Clin. Chem., March 1, 1998; 44(3): 639 - 648.
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N. N. Rehak, S. A. Cecco, J. E. Niemela, and R. J. Elin
Thiocyanate in smokers interferes with the Nova magnesium ion-selective electrode
Clin. Chem., September 1, 1997; 43(9): 1595 - 1600.
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