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Clinical Chemistry 36: 497-500, 1990;
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Clinical Chemistry, Vol 36, 497-500, Copyright © 1990 by American Association for Clinical Chemistry

Immunoadsorption of IgG onto second antibody covalently attached to Amino-Dylark beads for radioimmunoassays

SE Kakabakos, GP Evangelatos and DS Ithakissios
National Center for Scientific Research Demokritos, Athens, Greece.

We present a solid-phase immobilization method for radioligand assays, using an immunoadsorption coating procedure of anti-triiodothyronine rabbit IgG (anti-T3 IgG) onto second antibody (sheep anti-rabbit IgG) covalently bound to Amino-Dylark beads. The second antibody was in excess, compared with the first antibody, thus eliminating reproducibility problems between immunoadsorptions. Beads coated with second antibody can be used to immobilize a variety of antigen-specific first antibodies. The amount of anti-T3 antibody required for solid- phase T3 radioimmunoassay (RIA) was only 10% more, per assay tube, than that utilized in liquid-phase T3 RIA, in which polyethylene glycol solution was the separation reagent; characteristics of assay performance were comparable. The immobilization procedure requires high- titer antisera or antigen-specific IgG and seems advantageous because of the decrease in antibody requirements without significant modification of antibody functionality.




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