Clinical Chemistry
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Clinical Chemistry 36: 556-559, 1990;
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Clinical Chemistry, Vol 36, 556-559, Copyright © 1990 by American Association for Clinical Chemistry

3-Methylhistidine determined in plasma by "high-performance" lipid chromatography

HM van Eijk, NE Deutz, AJ Wagenmakers and PB Soeters
Department of Surgery, University of Limburg, Maastricht, The Netherlands.

In this fully automated method for determination of 3-methylhistidine (3MH) in plasma we use precolumn derivatization with o-phthaldialdehyde and subsequent separation by HPLC. Total analysis time is 36 min, and peak areas measured vary linearly with the amount of analyte injected, over the range of 0 to 20 pmol of 3MH (R2 = 0.995), with a coefficient of variation (CV) of 1.6%. The method is reliable, accurate, inexpensive, and at least 1000-fold more sensitive than conventional ion-exchange chromatography with ninhydrin. Because of its sensitivity, the method can be used to estimate venous-arterial differences. In four human volunteers the plasma 3MH concentration varied between 4.97 and 6.08 mumol/L, and the difference between "arterialized" and femoral venous blood for 3MH varied between 0.09 and 0.47 mumol/L.


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