Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 36: 559-561, 1990;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murthy, V. V.
Right arrow Articles by Karmen, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murthy, V. V.
Right arrow Articles by Karmen, A.

Clinical Chemistry, Vol 36, 559-561, Copyright © 1990 by American Association for Clinical Chemistry

A study of the CEDIA digoxin immunoassay

VV Murthy and A Karmen
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.

We evaluated the CEDIA digoxin immunoassay (Microgenics, Inc., Concord, CA), as performed with the Cobas-Bio centrifugal analyzer. In assays of sera with known concentrations of digoxin, the enzyme activity, measured when two beta-galactosidase (EC 3.2.1.23) fragments were combined according to the assay format, was proportional to the digoxin concentration. Results of assays of sera containing 0 to 3 micrograms of digoxin per liter correlated well when compared with an RIA method: CEDIA, microgram/L = 1.00 x RIA - 0.06 microgram/L (n = 90, r = 0.95, Sxy = 0.05). Duplicate assays of three control sera containing 0.8, 2.2, or 3.3 micrograms/L, each analyzed 20 times a day with each group of patients' samples, gave within-run CVs of 1-3% and day-to-day CVs of 3-12%. The reconstituted CEDIA reagents were stable for at least a month at 5 degrees C. As many as 25 samples and controls can be assayed in half the time needed to complete a similar number of RIA measurements with comparable results.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Association for Clinical Chemistry.