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Clinical Chemistry 36: 662-665, 1990;
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Clinical Chemistry, Vol 36, 662-665, Copyright © 1990 by American Association for Clinical Chemistry

Competitive heterogeneous enzyme immunoassay for theophylline by flow- injection analysis with electrochemical detection of p-aminophenol

EP Gil, HT Tang, HB Halsall, WR Heineman and AS Misiego
Department of Chemistry, University of Cincinnati, OH 45221-0172.

A competitive enzyme-linked immunoabsorbent assay based on the flow- injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).


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