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Clinical Chemistry 36: 666-669, 1990;
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Clinical Chemistry, Vol 36, 666-669, Copyright © 1990 by American Association for Clinical Chemistry

Polyclonal antibody-based enzyme-linked immunosorbent assay of alpha 1- acid glycoprotein

B Tissot, N Seta, G Durand and J Feger
Laboratoire de Biochimie A, Hopital Bichat, Paris, France.

This is a noncompetitive enzyme-linked immunosorbent assay for measuring low concentrations (2 to 100 micrograms/L) of human alpha 1- acid glycoprotein (AGP; orosomucoid). The method is based on a simple "sandwich" technique involving polyclonal rabbit antisera against AGP. Mean within-run and total (between-run) CVs were 6.2% and 9.7%, respectively. Analytical recovery, tested in various biological fluids, averaged 101%. The technique has been successfully applied to diluted biological fluids such as bronchoalveolar lavage, cerebrospinal and amniotic fluids, and hepatocyte-culture supernates. Because of its analytical validity and the commercial availability of the reagents, this assay is suitable for large-scale determinations of AGP concentrations in those biological fluids in which its concentration is relatively low.


The following articles in journals at HighWire Press have cited this article:


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 Drug Metab. Dispos.Home page
K. Nishi, N. Fukunaga, and M. Otagiri
CONSTRUCTION OF EXPRESSION SYSTEM FOR HUMAN {alpha}1-ACID GLYCOPROTEIN IN PICHIA PASTORIS AND EVALUATION OF ITS DRUG-BINDING PROPERTIES
Drug Metab. Dispos., October 1, 2004; 32(10): 1069 - 1074.
[Abstract] [Full Text] [PDF]


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