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Clinical Chemistry 36: 778-780, 1990;
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Clinical Chemistry, Vol 36, 778-780, Copyright © 1990 by American Association for Clinical Chemistry

Decreased storage stability of creatine kinase in a cardiac reperfusion solution

G Dunphy and D Ely
Department of Biology, University of Akron, OH 44325.

Creatine kinase (CK; EC 2.7.3.2) has been used as an indicator of myocardial cellular damage. In this study we used a Krebs-Henseleit (KH) solution to reperfuse isolated rat hearts after 24 h of cold preservation and collected the KH reperfusate for assay of CK to assess cellular damage. We wanted to determine the stability of CK in the KH solution at different cold-storage temperatures and albumin concentrations. CK activity (mean +/- SEM) after one week of refrigeration (5 degrees C) was 93% +/- 1% of control values, whereas CK activity in nitrogen-frozen (-200 degrees C) samples was only 1.6% +/- 1% of control values, and that in samples frozen at moderately low temperatures (-10 degrees C) was 63% +/- 1% of control values. To enhance stability, we added albumin at several concentrations (49, 25, 12, and 6 g/L) to reperfusion collections in which CK had been previously determined. Specimens were frozen (-10 degrees C), then re- analyzed for CK weekly for three weeks. CK activity was maintained (100% +/- 5%) only in samples containing 25 g/L or more albumin. These data suggest that refrigeration (5 degrees C) for one week maintains normal CK activity in KH solution; however, if prolonged storage is necessary, a stabilizer such as albumin (greater than or equal to 25 g/L) will maintain analyte stability in frozen storage (-10 degrees C) for at least three weeks.


The following articles in journals at HighWire Press have cited this article:


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C.J Zuurbier, O Eerbeek, P.T Goedhart, E.A Struys, N.M Verhoeven, C Jakobs, and C Ince
Inhibition of the pentose phosphate pathway decreases ischemia-reperfusion-induced creatine kinase release in the heart
Cardiovasc Res, April 1, 2004; 62(1): 145 - 153.
[Abstract] [Full Text] [PDF]

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 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Dunphy, H. W. Richter, M. Azodi, J. Weigand, F. Sadri, F. Sellke, and D. Ely
The effects of mannitol, albumin, and cardioplegia enhancers on 24-h rat heart preservation
Am J Physiol Heart Circ Physiol, May 1, 1999; 276(5): H1591 - H1598.
[Abstract] [Full Text] [PDF]


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