Clinical Chemistry
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Clinical Chemistry 36: 837-840, 1990;
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Clinical Chemistry, Vol 36, 837-840, Copyright © 1990 by American Association for Clinical Chemistry

Sensitive and rapid colorimetric immunoenzymometric assay of ferritin in biological samples

GA Ramm, LR Duplock, LW Powell and JW Halliday
Department of Medicine, Royal Brisbane Hospital, Australia.

We describe a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for quantifying ferritin in human and rat biological fluids. We used chlorophenol red beta-D-galactopyranoside as the colorimetric substrate of beta-galactosidase (EC 3.2.1.23), which is coupled to specific antibodies to either human or rat liver ferritin. The assay is sensitive (detection limit for human assay = 0.58 micrograms/L and for rat assay = 0.37 micrograms/L), accurate (average recovery for human assay = 93% and for rat assay = 92%), and precise (total CVs for human assay = 2.3-12.2% and for rat assay = 5.6-11.3%). The results correlated well with those of an established immunoradiometric technique (r = 0.99691). This assay has a prolonged shelf-life, is inexpensive, and utilizes a stable colorimetric substrate that requires relatively short incubation.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
P. Englebienne, A. Van Hoonacker, and J. Valsamis
Rapid Homogeneous Immunoassay for Human Ferritin in the Cobas Mira Using Colloidal Gold as the Reporter Reagent
Clin. Chem., December 1, 2000; 46(12): 2000 - 2003.
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