| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 36, 888-891, Copyright © 1990 by American Association for Clinical Chemistry
OC Boerman, MF Segers, LG Poels, P Kenemans and CM Thomas
Department of Cell Biology & Histology, University of Nijmegen, The Netherlands.
An in-house OC 125 monoclonal antibody-based "sandwich" immunofluorometric assay (IFMA) described previously (Clin Chem 1987; 33:2191-4) gave higher results for CA 125 in 37 of 123 serum samples than did a commercially available immunoradiometric assay (IRMA). Discordant results between the two assays became concordant when measurements of the samples were repeated with normal mouse serum (100 mL/L) included in the IFMA reagent. The murine immunoglobulins are thought to block the ability of the heterophilic antibodies in the human serum samples to cross-link the labeled antibody with the solid- phase antibody. Using an enzyme immunoassay, we demonstrated human anti- mouse antibodies (HAMA) in most of the discordant samples examined. We tested the ability of nonimmune sera from other animal species to lower the apparent CA 125 concentrations of the spurious samples and observed that rat, goat, and sheep serum were less effective than mouse serum. One serum sample was discovered to give a falsely increased CA 125 result with the IRMA, but this increase could be prevented by adding murine serum to the IRMA reagent. We conclude that falsely increased CA 125 results are best prevented by adding murine serum (or murine antibodies) to the assay buffer.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  T. B. Martins, B. M. Pasi, C. M. Litwin, and H. R. Hill Heterophile Antibody Interference in a Multiplexed Fluorescent Microsphere Immunoassay for Quantitation of Cytokines in Human Serum Clin. Vaccine Immunol., March 1, 2004; 11(2): 325 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A.A. Ismail, P. L. Walker, J. H. Barth, K. C. Lewandowski, R. Jones, and W. A. Burr Wrong Biochemistry Results: Two Case Reports and Observational Study in 5310 Patients on Potentially Misleading Thyroid-stimulating Hormone and Gonadotropin Immunoassay Results Clin. Chem., November 1, 2002; 48(11): 2023 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Bertholf, L. Johannsen, and G. Benrubi False Elevation of Serum CA-125 Level Caused by Human Anti-Mouse Antibodies Ann. Clin. Lab. Sci., October 1, 2002; 32(4): 414 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Bidart, F. Thuillier, C. Augereau, J. Chalas, A. Daver, N. Jacob, F. Labrousse, and H. Voitot Kinetics of Serum Tumor Marker Concentrations and Usefulness in Clinical Monitoring Clin. Chem., October 1, 1999; 45(10): 1695 - 1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Kricka Human Anti-Animal Antibody Interferences in Immunological Assays Clin. Chem., July 1, 1999; 45(7): 942 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Fitzmaurice, C. Brown, N. Rifai, A. H. B. Wu, and K.-T. J. Yeo False Increase of Cardiac Troponin I with Heterophilic Antibodies Clin. Chem., October 1, 1998; 44(10): 2212 - 2214. [Full Text] [PDF]
|
|
|
|
|
|
N. Desem and S. L. Jones Development of a Human Gamma Interferon Enzyme Immunoassay and Comparison with Tuberculin Skin Testing for Detection of Mycobacterium tuberculosis Infection Clin. Vaccine Immunol., July 1, 1998; 5(4): 531 - 536. [Abstract] [Full Text]
|
|
|
|
|
|
N. Despres and A. M. Grant Antibody interference in thyroid assays: a potential for clinical misinformation Clin. Chem., March 1, 1998; 44(3): 440 - 454. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
