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Clinical Chemistry 36: 1305-1311, 1990;
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Clinical Chemistry, Vol 36, 1305-1311, Copyright © 1990 by American Association for Clinical Chemistry

Specific, sensitive, precise, and rapid functional chromogenic assay of activated first complement component (C1) in plasma

S Munkvad, J Jespersen, J Sidelmann and J Gram
Department of Clinical Chemistry, Ribe County Hospital, Esbjerg, Denmark.

We present a new functional assay for the first complement component (C1) in plasma, based on its activation by inhibition of the C1- esterase inhibitor (C1-inh) when monospecific antiserum to C1-inh is added to the plasma. After maximal activation, we can determine the concentration of activated C1 by using an amidolytic rate assay with a chromogenic substrate. We have optimized the assay conditions with respect to incubation time, concentration of antiserum to C1-inh, ionic strength, and pH. Our method determines specifically the concentration in plasma of free activated C1, not complexes of activated C1 with C1- inh, and is not influenced by the concentration of C1-inh in the test sample. Concentrations of C1 correlated significantly with activities determined by a hemolytic assay (r = 0.55, t = 4.09, P less than 0.001). The estimated interassay CV was 5% and the intra-assay CV was 1%. The sensitivity, imprecision, and practical test performance of our assay are superior to those of conventionally used hemolytic assays.




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Copyright © 1990 by the American Association for Clinical Chemistry.