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Clinical Chemistry 36: 1333-1338, 1990;
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Clinical Chemistry, Vol 36, 1333-1338, Copyright © 1990 by American Association for Clinical Chemistry

Interference by human anti-mouse antibodies in CA 125 assay after immunoscintigraphy: anti-idiotypic antibodies not neutralized by mouse IgG but removed by chromatography

U Turpeinen, P Lehtovirta, H Alfthan and UH Stenman
Helsinki University Central Hospital, Department of Obstetrics and Gynecology, Finland.

Falsely increased concentrations of the ovarian carcinoma-associated antigen, CA 125, were measured by a monoclonal antibody (MAb)-based double determinant immunoradiometric assay (IRMA) in patients who developed antibodies to mouse immunoglobulins (IgGs) after receiving injections of the same MAb as is used in the CA 125 IRMA. Addition of undiluted mouse serum or purified mouse IgG to the assay mixture failed to eliminate the falsely increased CA 125 concentrations in most of the samples, owing to the presence of anti-idiotype antibody. Because of their anti-idiotypic nature, the human anti-mouse antibodies (HAMAS) had only little effect on other immunometric assays, and this effect could be completely eliminated by addition of mouse IgG. To eliminate the effect of HAMA on the CA 125 assay, we studied the ability of various chromatographic methods to separate the interfering HAMA from CA 125. For measuring HAMA in serum and chromatographic fractions we developed a time-resolved fluoroimmunoassay. Adequate separation of CA 125 and HAMA was achieved by affinity chromatography of patients' sera with solid-phase Protein A, Protein G, cation-exchange chromatography on Mono S, and gel filtration on Superose 6. These results demonstrate that the interference can effectively be removed by rather simple chromatographic procedures.


The following articles in journals at HighWire Press have cited this article:


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 Annals of Clinical & Laboratory ScienceHome page
R. L. Bertholf, L. Johannsen, and G. Benrubi
False Elevation of Serum CA-125 Level Caused by Human Anti-Mouse Antibodies
Ann. Clin. Lab. Sci., October 1, 2002; 32(4): 414 - 418.
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J. Bjerner, K. Nustad, L. F. Norum, K. H. Olsen, and O. P. Bormer
Immunometric Assay Interference: Incidence and Prevention
Clin. Chem., April 1, 2002; 48(4): 613 - 621.
[Abstract] [Full Text] [PDF]

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M. Covinsky, O. Laterza, J. D. Pfeifer, T. Farkas-Szallasi, and M. G. Scott
An IgM {lambda} Antibody to Escherichia coli Produces False-Positive Results in Multiple Immunometric Assays
Clin. Chem., August 1, 2000; 46(8): 1157 - 1161.
[Abstract] [Full Text] [PDF]

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L. J. Kricka
Human Anti-Animal Antibody Interferences in Immunological Assays
Clin. Chem., July 1, 1999; 45(7): 942 - 956.
[Abstract] [Full Text] [PDF]


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