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Clinical Chemistry 36: 1369-1372, 1990;
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Clinical Chemistry, Vol 36, 1369-1372, Copyright © 1990 by American Association for Clinical Chemistry

Identification of an ascorbic acid metabolite among "uremic middle molecules"

PM Gallice, JP Monti, DL Braguer, HM Monti, RE Elsen, YF Berland and AD Crevat
Laboratoire de Biophysique, Faculte de Pharmacie, Marseille, France.

Among uremic toxins in the middle molecular mass range, 1H, 13C-nuclear magnetic resonance, ultraviolet spectrometry, and chromatographic analyses allow identification of the main component of the so-called "2- 5-3 fraction" as ascorbic acid 2-sulfate, a conjugated metabolite of ascorbic acid. We previously (Clin Nephrol 1986;25:212-8) showed an inhibitory effect of the 2-5-3 fraction on microtubule formation. Therefore, we tested the action of ascorbic acid 2-sulfate and its synthetized enantiomers on tubulin polymerization. Because these molecules did not exert any inhibitory effect, we hypothesize that the 2-5-3 fraction is a mixture of compounds in which only a very low quantity of the inhibitory factor is present.




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