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Clinical Chemistry 36: 1431-1435, 1990;
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Clinical Chemistry, Vol 36, 1431-1435, Copyright © 1990 by American Association for Clinical Chemistry

Differential electroimmunoassay of human LpA-I lipoprotein particles on ready-to-use plates

HJ Parra, H Mezdour, N Ghalim, JM Bard and JC Fruchart
Service de Recherches sur les Lipoproteines et l'Atherosclerose INSERM U325, Institut Pasteur, Lille, France.

We describe a method for directly measuring LpA-I lipoprotein particles containing apolipoprotein A-I (apo A-I) not associated with apolipoprotein A-II (apo A-II), by differential electroimmunoassay of plasma on ready-to-use plates. Lipoprotein particles containing both apo A-I and apo A-II (LpA-I:A-II) are retained close to the wells when a very high excess of anti-apo A-II is used as compared with anti-apo A- I, whereas the LpA-I particles migrate and react with anti-apo A-I. The method is specific, rapid, and precise. Within- and between-run CVs at three concentrations (high, medium, and low) ranged between 1.51% and 2.72% and 3.01% and 4.56%, respectively. Analytical recovery of isolated LpA-I was from 93% to 115%. Results correlate well with those obtained by two-phase electroimmunoassay, enzyme-linked differential- antibody immunosorbent assay, and immunoaffinity chromatography coupled to enzyme-linked immunosorbent assay. The average normolipidemic concentration of LpA-I was 600 mg/L in 45 women and 490 mg/L in 40 men (P less than 0.0001).


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Copyright © 1990 by the American Association for Clinical Chemistry.