Clinical Chemistry
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Clinical Chemistry 36: 1494-1497, 1990;
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Clinical Chemistry, Vol 36, 1494-1497, Copyright © 1990 by American Association for Clinical Chemistry

Optimized fluorometric determination of urinary delta-aminolevulinic acid by using pre-column derivatization, and identification of the derivative

A Okayama, S Fujii and R Miura
Department of Health Science, Shiga University of Medical Science, Japan.

We previously reported the usefulness of a fluorometric method to determine urinary delta-aminolevulinic acid (ALA) concentrations by using post-column derivatization to monitor the effect of lead exposure. We have further improved the method by introducing pre-column derivatization by using reaction of ALA with acetylacetone and formaldehyde. Response of the hematopoietic system to lead exposure can now be easily detected at blood lead concentrations as low as 162 micrograms/L. The fluorescent ALA derivative, a new aromatic product, 2- methylideneamino-3,5-diacetyl-4,6-dimethylphenylpropionic acid, is separated on octadecyl silica column by high-performance liquid chromatography and the fluorescence intensity is detected with a fluorophotometer. Sample recoveries for 12 urine samples from workers exposed to lead and unexposed controls were 91.9-110.2%. The results obtained by the pre-column derivatization method agreed with those by the post-column derivatization method. The new method increases the sensitivity to a detection limit to 10 micrograms of delta- aminolevulinic acid per milliliter of urine and is simple enough to be used for routine monitoring of the biological effect of exposure to low concentrations of lead.


The following articles in journals at HighWire Press have cited this article:


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 Hum Exp ToxicolHome page
H. G. Kang, S. H. Jeong, M. R. Cho, J. H. Cho, and K. Bischoff
Time-dependent changes in lead and {delta}-aminolevulinic acid after subchronic lead exposure in rats
Human and Experimental Toxicology, October 1, 2009; 28(10): 647 - 654.
[Abstract] [PDF]

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 Drug Metab. Dispos.Home page
J. Meyer, M. C. Meyer, and A. L. Golub
Pharmacokinetics of Aminolevulinic Acid after Oral and Intravenous Administration in Dogs
Drug Metab. Dispos., April 1, 1999; 27(4): 432 - 435.
[Abstract] [Full Text]


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