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Clinical Chemistry 37: 14-19, 1991;
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Clinical Chemistry, Vol 37, 14-19, Copyright © 1991 by American Association for Clinical Chemistry

Capillary electrophoresis--a new clinical tool

FT Chen, CM Liu, YZ Hsieh and JC Sternberg
Applied Research Department, Beckman Instruments Inc., Brea, CA 92621.

The potential clinical diagnostic utility of capillary electrophoresis in an open-tubular column is established. Separation patterns for serum proteins by conventional agarose gel electrophoresis can be faithfully reproduced by a capillary electrophoresis procedure that provides the complete run data in 8 min. Hemoglobin variants can be separated within 10 min. The capillary electrophoresis separations are performed reliably and reproducibly in an untreated 75 micron (i.d.) x 25 cm fused-silica column. Diluted serum or hemoglobin samples can be loaded on an automated instrument with on-line injection, detection, and quantification, providing a truly "walkaway" electrophoresis system.


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Copyright © 1991 by the American Association for Clinical Chemistry.