Clinical Chemistry
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Clinical Chemistry 37: 20-25, 1991;
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Clinical Chemistry, Vol 37, 20-25, Copyright © 1991 by American Association for Clinical Chemistry

Microparticle-enhanced nephelometric immunoassay (Nephelia) for immunoglobulins G, A, and M

ML Cuilliere, P Montagne, T Bessou, R el Omari, D Riochet, P Varcin, P Laroche, P Prud'homme, J Marchand and O Flecheux
Immunology Laboratory, Faculty of Medicine, Vandoeuvre-les-Nancy, France.

Covalent binding of gamma chains of IgG, whole IgA, and mu chains of IgM on polyfunctional hydrophilic microspheres (MS) yields MS-Ig conjugates, usable as reagents in new microparticle-enhanced nephelometric immunoassays (Nephelia). The principle of the assays is inhibition by free analyte (IgG, IgA, and IgM) of agglutination of the MS-Ig conjugate with specific antiserum, the light scattered by the aggregates being measured with a nephelometer. The immunoglobulin assays developed are easy to perform (single-step assays, no washing or phase separation) and sensitive (high dilution of biological samples to exclude interferences and pretreatment). Analytical recovery results (95.4-101.2%) and correlations with generally used commercial assays (r = 0.86-0.98) indicate that the assays are accurate for large concentration ranges of immunoglobulins. Precision study gives CVs = 2.8-9.6%. Nephelia appears to be useful for quantifying a large variety of biological molecules.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
P. Montagne, M. L. Cuilliere, C. Mole, M. C. Bene, and G. Faure
Microparticle-enhanced nephelometric immunoassay of lysozyme in milk and other human body fluids
Clin. Chem., August 1, 1998; 44(8): 1610 - 1615.
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Copyright © 1991 by the American Association for Clinical Chemistry.