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Clinical Chemistry 37: 1742-1745, 1991;
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Clinical Chemistry, Vol 37, 1742-1745, Copyright © 1991 by American Association for Clinical Chemistry

Quantification of serum amyloid P by enzyme-linked immunosorbent assay

R Saile, A Kandoussi, M Deveaux, J Descamps, E Hachulla and C Cachera
Laboratoire de Recherche Nephrologique, Hopital Calmette, Lille, France.

This enzyme-linked immunosorbent assay procedure for quantifying serum amyloid P (SAP) in human plasma makes use of affinity-purified polyclonal antibodies to SAP in a "sandwich"-type format. The procedure is sensitive, reproducible, simple, and easily automatable. Results correlate well with those by a rocket immunoelectrophoresis method performed with the same antibodies. Sera from apparently normal individuals had a mean SAP content of 44.17 mg/L and increased with age.




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Copyright © 1991 by the American Association for Clinical Chemistry.