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Clinical Chemistry 37: 1913-1916, 1991;
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Clinical Chemistry, Vol 37, 1913-1916, Copyright © 1991 by American Association for Clinical Chemistry

New method for isolating and quantifying intermediate and beta-very-low- density lipoprotein cholesterol

RL Wikinski, LE Schreier and SB Rosental
Department of Clinical Biochemistry, University of Buenos Aires, Argentina.

We describe a new method, useful to clinical laboratories, for assessing intermediate density (IDL) or beta-very-low-density (beta- VLDL) lipoprotein cholesterol. The technique involves selective precipitation properties of the qualitative Wieland and Seidel post- electrophoretic method that immobilizes IDL and beta-VLDL in the beta- zone of an agarose slide (Clin Chem 1973;19:1139-41). In our method, we separate low-density lipoprotein (LDL) in a second electrophoretic step, in which LDL moves toward the anode, and then quantify the cholesterol of the above lipoproteins remaining in the precipitate band at the beta-zone. Replicate within-run precision (CV) of 15 aliquots of a sera pool was 10.1%. The correlation with sequential ultracentrifugation of 30 samples was r = 0.96 (P less than 0.001). Serum reference values for 30 normal individuals are 57 +/- 7.0 mg/L. Seven phenotype III hyperlipoproteinemic patients had the highest concentrations of IDL or beta-VLDL cholesterol in serum, 1620 +/- 346 mg/L.


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Copyright © 1991 by the American Association for Clinical Chemistry.