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Clinical Chemistry 37: 1978-1980, 1991;
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Clinical Chemistry, Vol 37, 1978-1980, Copyright © 1991 by American Association for Clinical Chemistry

Lactate analyzer with continuous blood sampling for monitoring blood lactate during physical exercise

N Shimojo, K Fujino, S Kitahashi, M Nakao, K Naka and K Okuda
Department of Laboratory Medicine, Osaka City University Medical School, Japan.

To monitor changes in the concentration of blood lactate during physical exercise, we used an automated lactate analyzer based on an electro-enzymatic method with continuous blood sampling through a catheter. The lactate concentration was measured every 2 min; between measurements, the instrument was calibrated with a lactate standard. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere with the measurements. The lactate concentrations in blood samples from apparently healthy subjects before and after exercise correlated well (r = 0.993) with results by the conventional enzymatic method. We measured the blood lactate concentrations in nine apparently healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. Thus, the lactate analyzer is suitable for monitoring changes in blood lactate concentrations during exercise.




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Copyright © 1991 by the American Association for Clinical Chemistry.