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Clinical Chemistry 37: 1981-1982, 1991;
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Clinical Chemistry, Vol 37, 1981-1982, Copyright © 1991 by American Association for Clinical Chemistry

Rapid, semi-quantitative assay of C-reactive protein evaluated

H Vallance and G Lockitch
Department of Pathology, University of British Columbia, Vancouver, Canada.

We evaluated a new rapid semi-quantitative immunometric assay of C- reactive protein (CRP) as a screening test for sepsis by comparison with an automated nephelometric method. Plasma samples (n = 101) from preterm infants during the first week of life were saved for CRP analyses. We measured CRP by the Nycocard semiquantitative method and compared the results with those obtained with a Behring Nephelometer. A CRP value less than 10 mg/L was considered to be negative for infection. All CRP results read as less than 10 mg/L (negative) by the Nycocard method were also less than 10 mg/L by the comparison method, and all CRP values found to be greater than 20 mg/L (positive) by the Nycocard method were also positive by the comparison method. Results in the 10-20 mg/L range were considered equivocal. We conclude that the Nycocard CRP semi-quantitative method is a rapid and useful screening test for sepsis in preterm infants.





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Copyright © 1991 by the American Association for Clinical Chemistry.