Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 37: 1987-1989, 1991;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stabler, T. V.
Right arrow Articles by Siegel, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stabler, T. V.
Right arrow Articles by Siegel, A. L.

Clinical Chemistry, Vol 37, 1987-1989, Copyright © 1991 by American Association for Clinical Chemistry

Chemiluminescence immunoassay of aldosterone in serum

TV Stabler and AL Siegel
Department of Pathology, University of Alabama, Birmingham 35294.

A chemiluminescence immunoassay (CLI) for the direct measurement of aldosterone in serum was developed with aminobutylethyl isoluminol (ABEI) as the label. In this competitive assay the samples are incubated with sample, antibody, aldosterone-carboxymethyl oxime-ABEI, and paramagnetic particles coated with second antibody. After magnetic separation and washing, the samples are incubated with 200 microL of NaOH (2 mol/L) at 60 degrees C for 30 min. In the luminometer the chemiluminescence is produced by the serial injection of 150 microL each of microperoxidase and H2O2 solutions. Comparison of results with an RIA method showed excellent agreement: CLI = 1.001 RIA + 0.020 (r = 0.99, n = 93). The method is simple and avoids the hazards and costs associated with isotopic waste. The label has a shelf life of at least two years.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C. Schirpenbach, L. Seiler, C. Maser-Gluth, F. Beuschlein, M. Reincke, and M. Bidlingmaier
Automated Chemiluminescence-Immunoassay for Aldosterone during Dynamic Testing: Comparison to Radioimmunoassays with and without Extraction Steps
Clin. Chem., September 1, 2006; 52(9): 1749 - 1755.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Association for Clinical Chemistry.