| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 37, 1989-1993, Copyright © 1991 by American Association for Clinical Chemistry
SL Perkins, JF Livesey, EA Escares, JM Belcher and DK Dudley
Department of Laboratory Medicine, Ottawa Civic Hospital, Canada.
Cotinine is a sensitive and specific biochemical marker of exposure to cigarette smoke. We describe a simple solid-phase extraction of cotinine from plasma before quantification by HPLC. Extraction recovery was 97.9% +/- 11.0% for plasma concentrations of 5-400 micrograms/L. Baseline separation of cotinine and caffeine was achieved within 11 min of injection onto a C18 reversed-phase column. The mobile phase was citric acid/dibasic potassium phosphate (30 mmol/L each, pH 6.0) containing 100 mL of acetonitrile per liter. Within-day and day-to-day precision (CV) were 4.7% and 8.4%, respectively. We also describe a modification of the Nicotine Metabolite RIA kit (Diagnostic Products Corp.) for quantifying cotinine in plasma. Recovery of cotinine from supplemented plasma was within 10% of the expected value with this RIA kit. Interassay precision averaged 8.1% for samples in the range 50-400 micrograms/L; intra-assay precision averaged 3.6% at 230 micrograms/L and 8.7% at 53 micrograms/L. Correlation between the two methods was RIA = 1.13 HPLC + 14.8 (n = 128, r = 0.957, P less than 0.001). Both methods are technically simple to perform.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  J. S LINDHOLT Activators of Plasminogen and the Progression of Small Abdominal Aortic Aneurysms Ann. N.Y. Acad. Sci., November 1, 2006; 1085(1): 139 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lodovici, S. Caldini, C. Luceri, F. Bambi, V. Boddi, and P. Dolara Active and Passive Smoking and Lifestyle Determinants of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Levels in Human Leukocyte DNA Cancer Epidemiol. Biomarkers Prev., December 1, 2005; 14(12): 2975 - 2977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Johnson, N. Ait-Daoud, F. Z. Akhtar, and M. A. Javors Use of Oral Topiramate to Promote Smoking Abstinence Among Alcohol-Dependent Smokers: A Randomized Controlled Trial Arch Intern Med, July 25, 2005; 165(14): 1600 - 1605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Korpilahde, M. Heliovaara, P. Knekt, J. Marniemi, A. Aromaa, and K. Aho Smoking history and serum cotinine and thiocyanate concentrations as determinants of rheumatoid factor in non-rheumatoid subjects Rheumatology, November 1, 2004; 43(11): 1424 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D HUTCHINSON, R MOOTS, M HELIÖVAARA, K AHO, P KNEKT, A REUNANEN, A AROMAA, and O IMPIVAARA Coffee consumption, RF, and the risk of RA Ann Rheum Dis, May 1, 2001; 60(5): 540a - 541. [Full Text]
|
|
|
|
 |
|
 P. Patrignani, M. R. Panara, S. Tacconelli, F. Seta, T. Bucciarelli, G. Ciabattoni, P. Alessandrini, A. Mezzetti, G. Santini, M. G. Sciulli, et al. Effects of Vitamin E Supplementation on F2-Isoprostane and Thromboxane Biosynthesis in Healthy Cigarette Smokers Circulation, August 1, 2000; 102(5): 539 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Bernert Jr., W. E. Turner, J. L. Pirkle, C. S. Sosnoff, J. R. Akins, M. K. Waldrep, Q. Ann, T. R. Covey, W. E. Whitfield, E. W. Gunter, et al. Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry Clin. Chem., December 1, 1997; 43(12): 2281 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Reilly, N. Delanty, J. A. Lawson, and G. A. FitzGerald Modulation of Oxidant Stress In Vivo in Chronic Cigarette Smokers Circulation, July 1, 1996; 94(1): 19 - 25. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
