| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 37, 2102-2110, Copyright © 1991 by American Association for Clinical Chemistry
R Libert, D Hermans, JP Draye, F Van Hoof, E Sokal and E de Hoffmann
Department of Neuropediatry, Cliniques Universitaires St. Luc, Brussels, Belgium.
From a study of the collision-activated fragmentation of bile acids, a qualitative analytical method based on negative ion fast atom bombardment tandem mass spectrometry has been developed. The times for sample preparation and analyses are short. Both free and conjugated bile acids are detected as they occur in biological fluids, without derivatization. For identifying bile acids and conjugates, the method offers better specificity and sensitivity than does the fast atom bombardment mass spectrometric technique alone. Specific scan modes have been developed for the selective detection of taurine conjugates, delta 4-unsaturated taurine conjugates, delta 4-3-keto free acids and their glycine conjugates, free acids and glycine conjugates bearing a hydroxyl group at the C-12 position, sulfates of glycine and taurine conjugates, and a C29 dicarboxylic bile acid, specific for generalized peroxisomal disorders. Applications of this technique demonstrate its potential usefulness, principally in the diagnosis of several peroxisomal disorders.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  Y. Alnouti Bile Acid Sulfation: A Pathway of Bile Acid Elimination and Detoxification Toxicol. Sci., April 1, 2009; 108(2): 225 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wang, M. Salem, I. M. Yousef, B. Tuchweber, P. Lam, S. J. Childs, C. D. Helgason, C. Ackerley, M. J. Phillips, and V. Ling Targeted inactivation of sister of P-glycoprotein gene (spgp) in mice results in nonprogressive but persistent intrahepatic cholestasis PNAS, February 1, 2001; (2001) 31465498. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. W. Johnson, H. J. ten Brink, R. C. Schuit, and C. Jakobs Rapid and quantitative analysis of unconjugated C27 bile acids in plasma and blood samples by tandem mass spectrometry J. Lipid Res., January 1, 2001; 42(1): 9 - 16. [Abstract] [Full Text]
|
|
|
|
|
|
S. Perwaiz, B. Tuchweber, D. Mignault, T. Gilat, and I. M. Yousef Determination of bile acids in biological fluids by liquid chromatography-electrospray tandem mass spectrometry J. Lipid Res., January 1, 2001; 42(1): 114 - 119. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Baes, S. Huyghe, P. Carmeliet, P. E. Declercq, D. Collen, G. P. Mannaerts, and P. P. Van Veldhoven Inactivation of the Peroxisomal Multifunctional Protein-2 in Mice Impedes the Degradation of Not Only 2-Methyl-branched Fatty Acids and Bile Acid Intermediates but Also of Very Long Chain Fatty Acids J. Biol. Chem., May 19, 2000; 275(21): 16329 - 16336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wang, M. Salem, I. M. Yousef, B. Tuchweber, P. Lam, S. J. Childs, C. D. Helgason, C. Ackerley, M. J. Phillips, and V. Ling Targeted inactivation of sister of P-glycoprotein gene (spgp) in mice results in nonprogressive but persistent intrahepatic cholestasis PNAS, February 13, 2001; 98(4): 2011 - 2016. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
