Clinical Chemistry
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Clinical Chemistry 37: 226-230, 1991;
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Clinical Chemistry, Vol 37, 226-230, Copyright © 1991 by American Association for Clinical Chemistry

Practical method for optimizing radioimmunoassay detection and precision limits

E Ezan, C Tiberghien and F Dray
Institut Pasteur, Unite de Radioimmunologie Analytique/INSERM U207, Paris, France.

A model of the competitive radioimmunoassay standard curve, based on the Law of Mass Action, has been developed and used in conjunction with experimental and counting errors to predict the assay detection limit and precision profiles. We verified the model with hapten radioimmunoassays performed in our laboratory. The resulting computer program can be used to determine the optimum antiserum concentration-- depending on its affinity--and labeled-antigen concentration--according to its specific activity and nonspecific binding. The graphical representation of this model provides radioimmunologists with a practical tool for assay optimization.




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Copyright © 1991 by the American Association for Clinical Chemistry.