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Clinical Chemistry 37: 281-284, 1991;
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Clinical Chemistry, Vol 37, 281-284, Copyright © 1991 by American Association for Clinical Chemistry

Osteocalcin concentrations in plasma prepared with different anticoagulants

MJ Power, B O'Dwyer, E Breen and PF Fottrell
Department of Biochemistry, University College Hospital, Galway, Ireland.

We investigated the effects on plasma osteocalcin concentrations of different anticoagulants used to collect the blood samples. Plasma osteocalcin concentrations measured by enzyme immunoassay and radioimmunoassay are influenced by the nature of the anticoagulants used. The most significant difference between concentrations found in plasma and serum was seen with oxalate/fluoride anticoagulant, which reduced osteocalcin concentrations to 37.3% of serum values. This is probably related to increased hemolysis with this anticoagulant compared with osteocalcin concentrations in plasma prepared with other anticoagulants. Samples prepared with sodium citrate (0.105 mol/L) or lithium heparin gave values 92.4% and 83.6% of those obtained with matched serum samples. Osteocalcin concentrations were relatively stable in plasma and serum at -20 degrees C for two freeze/thaw cycles. In blood from 100 patients there was a good correlation between osteocalcin concentrations in serum and plasma (lithium heparin) (r2 = 0.831); the slope and intercept (+/- SE) were 0.924 +/- 0.04 and 4.92 +/- 1.25 micrograms/L, respectively. However, in 10 patients, serum osteocalcin concentrations were two- to threefold higher than those in matched plasma samples.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
J. W. Colford, B. A. Lueddecke, M. Salvati, D. Hanna, D. Sailer, S. Khosla, B. L. Riggs, and C. B. Langman
Immunoradiometric Assay for Intact Human Osteocalcin(1–49) without Cross-Reactivity to Breakdown Products
Clin. Chem., April 1, 1999; 45(4): 526 - 531.
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Copyright © 1991 by the American Association for Clinical Chemistry.