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Clinical Chemistry 37: 411-414, 1991;
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Clinical Chemistry, Vol 37, 411-414, Copyright © 1991 by American Association for Clinical Chemistry

Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors

A Larsson, A Karlsson-Parra and J Sjoquist
Department of Medical and Physiological Chemistry, Biomedical Center, Uppsala, Sweden.

Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to give a false- positive reaction. We show that rheumatoid factors do not react with chicken IgY; if the capture antibody or detection antibody (or both) is of avian origin, the interference of RF or other anti-IgG antibodies in sandwich ELISA can be avoided.


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Copyright © 1991 by the American Association for Clinical Chemistry.