Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 37: 536-539, 1991;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harthe, C.
Right arrow Articles by Chazot, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harthe, C.
Right arrow Articles by Chazot, G.

Clinical Chemistry, Vol 37, 536-539, Copyright © 1991 by American Association for Clinical Chemistry

Direct radioimmunoassay of 6-sulfatoxymelatonin in plasma with use of an iodinated tracer

C Harthe, B Claustrat, J Brun and G Chazot
Service de Radiopharmacie et Radioanalyse, Centre de Medecine Nucleaire, Lyon, France.

We describe here a direct a radioimmunoassay (RIA) for the determination of 6-sulfatoxymelatonin (aMT6s) in plasma, with iodinated aMT6s as tracer. The aMT6s antiserum was raised in rabbit by immunization with a bovine serum albumin conjugate, giving negligible cross-reactivities for related compounds. The low limit of detection (15 pmol/L) allowed a direct assay that required only a 100-microL plasma sample. Dilutions of plasma and of synthetic aMT6s gave the same parallel response in the RIA. A preliminary study showed a circadian variation in healthy volunteers, with mean concentrations ranging from 52 (at 1600-2100 h) to 378 pmol/L (at 0400 h), whereas this rhythm was abolished in pinealomectomized patients. After administration of melatonin orally, or by infusion, the aMT6s concentrations in plasma concorded with previous data on aMT6s production and pharmacokinetics, with aMT6s being cleared from plasma more slowly than melatonin. This assay should have practical application in the development of new pharmaceutical formulations that minimize the hepatic metabolism of melatonin.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Nutr.Home page
I. Fournier, F. Ploye, J.-M. Cottet-Emard, J. Brun, and B. Claustrat
Folate Deficiency Alters Melatonin Secretion in Rats
J. Nutr., September 1, 2002; 132(9): 2781 - 2784.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. Zaouali-Ajina, A. Gharib, G. Durand, N. Gazzah, B. Claustrat, C. Gharib, and N. Sarda
Dietary Docosahexaenoic Acid-Enriched Phospholipids Normalize Urinary Melatonin Excretion in Adult (n-3) Polyunsaturated Fatty Acid-Deficient Rats
J. Nutr., November 1, 1999; 129(11): 2074 - 2080.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Association for Clinical Chemistry.