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Clinical Chemistry 37: 563-565, 1991;
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Clinical Chemistry, Vol 37, 563-565, Copyright © 1991 by American Association for Clinical Chemistry

Determination of creatinine in serum and urine by cation-exchange high- pressure liquid chromatography

A Harmoinen, P Sillanaukee and H Jokela
Department of Clinical Chemistry, Tampere University Hospital, Finland.

We describe an HPLC method for quantifying creatinine, separating the analyte from other compounds in serum and urine by cation-exchange chromatography and measuring its absorbance at 234 nm. The precision of the method (CV) varied from 2.9% (mean creatinine concentration, 31 mumol/L) to 1.7% (361 mumol/L) within a series of assays and from 3.9% (34 mumol/L) to 2.4% (391 mumol/L) between series. A comparison with the Jaffe method, as performed with a Technicon SMA analyzer, gave the regression line yHPLC = 1.00xJaffe - 12.0 (n = 141, r = 0.998, and Syx = 19). Results also are comparable with those of an enzymatic method, if the enzymatic method is standardized with a serum-based standard when serum samples are measured. An aqueous standard has to be used for enzymatic determination of creatinine in urine.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
A. Harmoinen, T. Lehtimaki, M. Korpela, V. Turjanmaa, and H. Saha
Diagnostic Accuracies of Plasma Creatinine, Cystatin C, and Glomerular Filtration Rate Calculated by the Cockcroft-Gault and Levey (MDRD) Formulas
Clin. Chem., July 1, 2003; 49(7): 1223 - 1225.
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