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Clinical Chemistry 37: 576-578, 1991;
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Clinical Chemistry, Vol 37, 576-578, Copyright © 1991 by American Association for Clinical Chemistry

Modified immunoblotting technique for phenotyping lipoprotein(a)

CM Huang, HG Kraft and RE Gregg
National Institutes of Health, Bethesda, MD 20892.

We describe a modified immunoblotting method for phenotyping lipoprotein(a) [Lp(a)]. This immunoblotting procedure uses commercially available reagents that have a long shelf life. The method is sensitive and takes only 18 microL of sera. Lp(a) phenotyping can be performed on sera stored at 4 degrees C for as much as a week or at -80 degrees C for as long as a year. In a study of 145 unrelated healthy subjects, we found Lp(a) allelic frequencies of LpF = 1.8%, LpB = 2.6%, LpS1 = 5.1%, LpS2 = 14.8%, LpS3 = 35.9%, LpS4 = 11.6%, and LpO = 28.0%.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
R. Couderc, K. Peoc'h, K. Valenti, M-E. Diop, S. Bailleul, L. Kienou, F. Ferriere, and J. Etienne
A Simple Electrophoretic Method for Phenotyping Apo(a): Phenotype Frequency in Healthy Subjects from Paris, France
Clin. Chem., May 1, 1998; 44(5): 1047 - 1050.
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Copyright © 1991 by the American Association for Clinical Chemistry.