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Clinical Chemistry 37: 643-646, 1991;
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Clinical Chemistry, Vol 37, 643-646, Copyright © 1991 by American Association for Clinical Chemistry

Rapid liquid-chromatographic method for simultaneous determination of plasma prednisone, prednisolone, and cortisol in pediatric renal- transplant recipients

JH McBride, DO Rodgerson, SS Park and AF Reyes
Department of Pathology, UCLA School of Medicine 90024-1713.

A rapid liquid-chromatographic method is described for simultaneously determining prednisone, prednisolone, and cortisol in plasma. Before chromatography, samples containing an internal standard (methylprednisolone) were extracted with use of Chem Elut (Analytichem) columns. After elution of the glucocorticosteroids, the eluates were dried and reconstituted in a mobile phase of tetrahydrofuran/water (25/75 by vol). Samples were injected onto a reversed-phase C18 column, and analysis time was 15.5 min. Measures of analytical performance were all acceptable, and the method was used to assess noncompliance in pediatric renal-transplant recipients who were receiving prednisone and cyclosporine. Of the 37 pediatric patients tested, five (13.5%) were identified as noncompliant. The method is simple, accurate, and precise, and other steroids and medications commonly given to transplant recipients do not interfere with it.




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Copyright © 1991 by the American Association for Clinical Chemistry.