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Clinical Chemistry 37: 673-677, 1991;
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Clinical Chemistry, Vol 37, 673-677, Copyright © 1991 by American Association for Clinical Chemistry

Simple enzyme immunoassay for the simultaneous measurement of whole choriogonadotropin molecules and free beta-subunits in sera of women with abnormal pregnancies or tumors of the reproductive system

MJ Choi, IS Choe, HK Kang, JS Lee and TW Chung
Genetic Engineering Center, Korea Institute of Science and Technology, Seoul.

A multiple enzyme immunoassay (multi-EIA) was developed to quantify whole-molecule human choriogonadotropin (w-hCG) and free hCG beta- subunits (hCG-beta) simultaneously. A clone of a specific monoclonal antibody was coupled to solid phase; two other clones of different monoclonal antibodies were conjugated to horseradish peroxidase (HRP; EC 1.11.1.7) and alkaline phosphatase (AP; EC 3.1.3.1), respectively. These two enzyme conjugates were mixed together to measure w-hCG or hCG- beta, depending on the selection of the enzyme substrate. To measure w- hCG and hCG-beta simultaneously, both enzyme substrates were used with the blended enzyme conjugates. The assay is simple and reproducible, and can be completed within 2 h with high specificity and sensitivity. We measured w-hCG and hCG-beta in the sera of women with abnormal pregnancies and in patients with tumors of the reproductive system, and observed different hCG-beta/w-hCG ratios in patients with various types of trophoblastic tumors.


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J. Wu, F. Yan, J. Tang, C. Zhai, and H. Ju
A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers
Clin. Chem., August 1, 2007; 53(8): 1495 - 1502.
[Abstract] [Full Text] [PDF]


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Copyright © 1991 by the American Association for Clinical Chemistry.